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Medium optimization for production of anti-streptococcal liamocins by Aureobasidium pullulans

Author:
Leathers, Timothy D., Skory, Christopher D., Price, Neil P.J., Nunnally, Melinda S.
Source:
Biocatalysis and Agricultural Biotechnology 2018 v.13 pp. 53-57
ISSN:
1878-8181
Subject:
Aureobasidium pullulans, Streptococcus, anti-infective agents, culture media, fungi, matrix-assisted laser desorption-ionization mass spectrometry, statistical analysis
Abstract:
Liamocins are antimicrobial compounds with specificity for Streptococcus spp., produced by certain strains of the fungus Aureobasidium pullulans. Recent studies have identified strains and culture medium for liamocin production. However, this culture medium has not previously been optimized for liamocin production. In this study, we employ one-factor-at-a-time and statistical methods to develop an optimized medium for liamocin production. Liamocin yields from optimized medium were nearly double those from standard medium, up to 22g liamocin/L. This represents the highest yields of liamocin reported to date. Furthermore, MALDI-TOF analyses show that liamocins produced from optimized medium are similar to those produced from standard medium.
Handle:
10113/5871063