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Comparison of direct in‐house cerebrospinal fluid cytology with commercial pathology results in dogs

Author:
Newton, P. L., Fry, D. R., Best, M. P.
Source:
The journal of small animal practice 2017 v.58 no.12 pp. 694-702
ISSN:
0022-4510
Subject:
animal pathology, cell biology, cerebrospinal fluid, dogs, erythrocyte count, nervous system diseases
Abstract:
OBJECTIVES: To investigate the correspondence between in‐house direct cytological assessment of cerebrospinal fluid and results from a commercial veterinary pathology laboratory. METHODS: Prospective inclusion of samples from dogs that were presented for investigation of suspected neurological disease and had cerebrospinal fluid samples submitted to a commercial pathology laboratory for analysis. A board‐certified veterinary pathologist assessed all cerebrospinal fluid samples, and a line smear was assessed in‐house by two observers. Nucleated cell count, red blood cell count and differential cell counts were recorded and compared. RESULTS: In‐clinic and commercial pathology nucleated cell counts and red blood cell counts were strongly correlated. In‐house line smear results were compared with the gold standard of a defined dichotomous rating of ‘increased nucleated cell count’ provided by the external pathology service. Sensitivity was 93% and specificity 80% for samples with at least two cells per linear field. CLINICAL APPLICATION: Although not a replacement for the assessment of cerebrospinal fluid samples by specialist veterinary pathologists, this method can provide rapid and clinically meaningful information before externally processed sample results are available.
Agid:
5872104