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Isolation and identification of 3-bromocarbazole-degrading bacteria Part B Pesticides, food contaminants, and agricultural wastes

Ma, Yun, Li, Zhiwei, Yuan, Mei, Chen, Linhua, Zhou, Shanshan
Journal of environmental science and health 2017 v.52 no.11 pp. 796-801
Stenotrophomonas, bacteria, biodegradation, bioremediation, carbazoles, gas chromatography-mass spectrometry, genes, metabolites, pH, polluted soils, ribosomal RNA
In this study, a bacterial strain, CH-1, capable of degrading 3-bromocarbazole (3-BCZ) was isolated from a polluted soil. Based on its physio-biochemical characteristics and 16S rRNA genes, strain CH-1 was identified as a Stenotrophomonas sp . Strain CH-1 was able to degrade 70% of 50 mg/L 3-BCZ within 8 d at pH 7.0 and 30°C in mineral salt medium (MSM). During the process, the main intermediate metabolite was identified as (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2, 4-dienoic by gas (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2,4-dienoic via gas chromatograph-mass spectrometry (GC-MS) analysis. The metabolite disappeared after 14 d, suggesting that the metabolite can also be degraded by strain CH-1. 3-BCZ is a new persistent organic pollutant. This is the first report of the biodegradation of 3-BCZ. The results indicated that strain CH-1 may be a promising bacterial candidate for the bioremediation of environments polluted with polyhalogenated carbazoles (PHCs).