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Registration of Purified Accessions for the U.S. Peanut Mini-Core Germplasm Collection

Chen, Charles Y., Barkley, Noelle A., Wang, Ming L., Holbrook, C. Corley, Dang, Phat M.
Journal of plant registrations 2014 v.8 pp. 77-85
Arachis hypogaea, Cercospora, Tomato spotted wilt virus, USDA, chemical analysis, chemical composition, disease resistance, genetic variation, germplasm releases, information sources, irrigated conditions, lesions (plant), peanuts, plant morphology, planting, seeds, wilting, Alabama, Georgia
Many accessions of the USDA peanut (Arachis hypogaea L.) germplasm collection are heterogeneous. Advances in genomics technology have highlighted the need for collections of homogeneous accessions. The objectives of this research were to purify accessions of the USDA mini-core collection and to characterize this collection for morphological traits. Twenty seeds of one hundred and four accessions of the peanut mini-core collection were planted at Dawson, GA in 2008 under irrigated conditions. The seeds from five phonotypical uniform plants were harvested, bulked, and planted in Headland, AL in 2009 to continue the purification process. In 2010, homogenous seeds of each accession were planted in Headland, AL to increase seed counts for chemical analysis and genotyping. Besides morphological characters and seed chemical composition, the accessions were evaluated for spotted wilt resistance caused by a tomato spotted wilt virus (TSWV) and early leaf spot resistance caused by Cercospora arachidicola (Hori) which is a major disease that significantly impact peanut production in the southeast. The morphological and chemical data, along with images of pod and seed traits were entered in the Germplasm Resources Information Network (GRIN) and detailed information can be found at: The results showed that after purification, these accessions not only preserved similar genetic variation in the collection, but also became more homogeneous compared to the original accessions. A small amount of seed of the purified accession can be obtained for research and breeding purposes through the National Plant Germplasm System (