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Engineered Lactic Acid Bacterium Lactococcus lactis Capable of Binding Antibodies and Tumor Necrosis Factor Alpha

Author:
Ravnikar, Matjaž, Štrukelj, Borut, Obermajer, Nataša, Lunder, Mojca, Berlec, Aleš
Source:
Applied and environmental microbiology 2010 v.76 no.20 pp. 6928-6932
ISSN:
0099-2240
Subject:
Lactococcus lactis, antibodies, binding capacity, gastric juice, inflammatory bowel disease, lactic acid bacteria, tumor necrosis factors
Abstract:
We have optimized the display of the B domain of staphylococcal protein A on the surface of Lactococcus lactis. The maximum binding capacity was estimated at 0.146 μg of antibody per 10⁸ cells and was sustained at 86% after treatment with simulated gastric juice. A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease.
Agid:
587770