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Genetic markers in Atlantic salmon, Salmo salar L., their potential for experimental studies of gene flow and adaptation

Aquaculture research 1994 v.25 no.S2 pp. 121-129
Salmo salar, alleles, aquaculture, autumn, biopsy, eggs, females, gene flow, genetic markers, genetic variation, genotyping, hatcheries, heterozygosity, homozygosity, isozymes, males, mortality, muscles, rivers, spawning, tissues
In autumn 1992, a number of farmed and wild Atlantic salmon, Salmo salar L., spawners were screened for genetic variability in isoenzymes expressed in low‐risk tissues. Samples of muscle and fin tissues were taken by biopsy shortly before spawning. In the Daleelv hatchery a male heterozygous for GPI‐3*105 allele was crossed with four females homozygous for the common allele from Matre aquaculture station. In the River Hålva a wild male heterozygous for a GPI‐1,2*160 allele was crossed with a female homozygous for the common allele. Another female from Matre aquaculture stations, heterozygous for the MDH‐3,4*85 allele was crossed with a male homozygous for the common allele. Mortality was recorded during the egg and fry stages. The families were sampled four times during 1993, each individual was genotyped, and length and weight of the various genotypes recorded and compared. In all families the observed genotypic distributions were in close agreement with the expected Mendelian ratios, even in one family where the mortality was over 95%. There were no differences in performance between the two genotypes in any of the families. The inheritance of the observed variability was verified, and it is concluded that the detected alleles have a potential as genetic markers in experimental studies of gene flow and adaptation.