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iTRAQ‐based identification of differentially expressed proteins related to growth in the swimming crab, Portunus trituberculatus

Ren, Xianyun, Yu, Xuan, Gao, Baoquan, Li, Jian, Liu, Ping
Aquaculture research 2017 v.48 no.6 pp. 3257-3267
DNA, Portunus trituberculatus, energy, farmed animal species, gene expression regulation, genetic background, heart, hepatopancreas, immune response, liquid chromatography, messenger RNA, muscles, protein synthesis, proteins, proteome, proteomics, tandem mass spectrometry, tissues, China
The swimming crab, Portunus trituberculatus, is an important farmed species in China, and it has been studied extensively, which requires more information on its genetic background. To date, information on the proteomics of P. trituberculatus is scarce. In this study, we used isobaric tags for relative and absolute quantitation (iTRAQ)‐based proteomics to investigate growth regulation in P. trituberculatus. Total proteins were isolated from five tissues (eyestalk, gill, heart, hepatopancreas and muscle). Equal quantities of protein from each tissue were pooled at the proteome level using the iTRAQ method. A total of 961 proteins were identified using liquid chromatography coupled with tandem mass spectrometry and de novo sequencing data. Using a 1.2‐fold change in expression as a physiologically significant benchmark, 30 differentially expressed proteins (DEPs) were found to undergo differential expression related to the growth of P. trituberculatus, and most of the growth‐related proteins, including those involved in metabolism, immune responses, DNA duplication and protein synthesis, were upregulated, indicating that conservation of energy is an important strategy to cope with growth. There was a high consistency between the expression levels determined using iTRAQ and mRNA, highlighting the high reproducibility of our proteomic approach and its great value in elucidating the molecular mechanisms of P. trituberculatus.