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Lysozyme activity of the Ruminococcus champanellensis cellulosome

Author:
Moraïs, Sarah, Cockburn, Darrell W., Ben‐David, Yonit, Koropatkin, Nicole M., Martens, Eric C., Duncan, Sylvia H., Flint, Harry J., Mizrahi, Itzhak, Bayer, Edward A.
Source:
Environmental microbiology 2016 v.18 no.12 pp. 5112-5122
ISSN:
1462-2912
Subject:
Ruminococcus, adhesion, bacteria, cellobiose, cellulosome, digestive system, glycosides, humans, keystone species, lignocellulose, lysozyme, pH, temperature
Abstract:
Ruminococcus champanellensis is a keystone species in the human gut that produces an intricate cellulosome system of various architectures. A variety of cellulosomal enzymes have been identified, which exhibit a range of hydrolytic activities on lignocellulosic substrates. We describe herein a unique R. champanellensis scaffoldin, ScaK, which is expressed during growth on cellobiose and comprises a cohesin module and a family 25 glycoside hydrolase (GH25). The GH25 is non‐autolytic and exhibits lysozyme‐mediated lytic activity against several bacterial species. Despite the narrow acidic pH curve, the enzyme is active along a temperature range from 2 to 85°C and is stable at very high temperatures for extended incubation periods. The ScaK cohesin was shown to bind selectively to the dockerin of a monovalent scaffoldin (ScaG), thus enabling formation of a cell‐free cellulosome, whereby ScaG interacts with a divalent scaffodin (ScaA) that bears the enzymes either directly or through additional monovalent scaffoldins (ScaC and ScaD). The ScaK cohesin also interacts with the dockerin of a protein comprising multiple Fn3 domains that can potentially promote adhesion to carbohydrates and the bacterial cell surface. A cell‐free cellulosomal GH25 lysozyme may provide a bacterial strategy to both hydrolyze lignocellulose and repel eventual food competitors and/or cheaters.
Agid:
5880064