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Deacetylation biocatalysis and elicitation by immobilized Penicillium canescens in Astragalus membranaceus hairy root cultures: towards the enhanced and sustainable production of astragaloside IV

Author:
Gai, Qing‐Yan, Jiao, Jiao, Luo, Meng, Wang, Wei, Yao, Li‐Ping, Fu, Yu‐Jie
Source:
Plant biotechnology journal 2017 v.15 no.3 pp. 297-305
ISSN:
1467-7644
Subject:
Astragalus membranaceus, Fourier transform infrared spectroscopy, Penicillium, astragalosides, biocatalysis, biocatalysts, biochemical pathways, cell walls, coculture, elicitors, gene expression, gene expression regulation, immobilized cells, lignification, roots, scanning electron microscopy
Abstract:
A novel biotechnology approach by combining deacetylation biocatalysis with elicitation of immobilized Penicillium canescens (IPC) in Astragalus membranaceus hairy root cultures (AMHRCs) was proposed for the elevated production of astragaloside IV (AG IV). The highest AG IV accumulation was achieved in 36‐day‐old AMHRCs co‐cultured with IPC for 60 h, which resulted in the enhanced production of AG IV by 14.59‐fold in comparison with that in control (0.193 ± 0.007 mg/g DW). Meanwhile, AG IV precursors were almost transformed to AG IV by IPC deacetylation. Moreover, expression of genes involved in AG IV biosynthetic pathway was significantly up‐regulated in response to IPC elicitation. Also, FTIR and SEM showed that cell wall lignification was enhanced following IPC treatment and root surface was likely to be IPC deacetylation site. Overall, dual roles of IPC (biocatalyst and elicitor) offered an effective and sustainable way for the mass production of AG IV in AMHRCs.
Agid:
5880712