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Different effects of anthocyanins and phenolic acids from wild blueberry (Vaccinium angustifolium) on monocytes adhesion to endothelial cells in a TNF‐α stimulated proinflammatory environment
- Del Bo', Cristian, Roursgaard, Martin, Porrini, Marisa, Loft, Steffen, Møller, Peter, Riso, Patrizia
- Molecular nutrition & food research 2016 v.60 no.11 pp. 2355-2366
- Vaccinium angustifolium, adhesion, anthocyanins, atherogenesis, blueberries, cell adhesion, cyanidin, delphinidin, endothelium, gallic acid, human umbilical vein endothelial cells, malvidin, metabolites, monocytes, syringic acid, tumor necrosis factor-alpha
- SCOPE: Monocyte adhesion to the vascular endothelium is a crucial step in the early stages of atherogenesis. This study aims to investigate the capacity of an anthocyanin (ACN) and phenolic acid (PA) rich fraction (RF) of a wild blueberry, single ACNs (cyanidin, malvidin, delphinidin) and related metabolites (protocatechuic, syringic, and gallic acid) to counteract monocytes (THP‐1) adhesion to human umbilical vein endothelial cells (HUVECs) in a tumor necrosis factor α (TNF‐α) mediated proinflammatory environment. METHODS AND RESULTS: HUVECs were incubated with different concentrations (from 0.01 to 10 μg/mL) of the compounds for 24 h. Labeled monocytic THP‐1 cells were added to HUVECs and their adhesion was induced by TNF‐α (100 ng/mL). ACN‐RF reduced THP‐1 adhesion to HUVECs with a maximum effect at 10 μg/mL (−33%). PA‐RF counteracted THP‐1 adhesion at 0.01, 0.1, and 1 μg/mL (−45, −48.7, and −27.6%, respectively), but not at maximum concentration. Supplementation with gallic acid reduced THP‐1 adhesion to HUVECs with a maximum effect at 1 μg/mL (−29.9%), while malvidin‐3‐glucoside and syringic acid increased the adhesion. No effect was observed for the other compounds. CONCLUSION: These results suggest that ACNs/PA‐RF may prevent atherogenesis while the effects of the single ACNs and metabolites are controversial and merit further exploration.