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Characterization of biosurfactants as insecticidal metabolites produced by Bacillus subtilis Y9

Yang, Si Young, Lim, Da Jung, Noh, Mi Young, Kim, Jin Cheol, Kim, Young Cheol, Kim, In Seon
Entomological research 2017 v.47 no.1 pp. 55-59
Bacillus subtilis, Myzus persicae, bacteria, biodegradability, biological control agents, biosurfactants, high performance liquid chromatography, insecticidal properties, integrated pest management, isomers, leucine, metabolites, plant protection, screening, surface tension, surfactin, valine
Biosurfactants have been used for crop protection in integrated pest management programs, as they are generally biodegradable and environmentally friendly. While screening for insecticidal bacteria against green peach aphid, Myzus persicae, we found that a Bacillus isolate, Y9, exhibited strong surface tension activity and produced insecticidal metabolites. The insecticidal metabolites with surface tension activity were extracted from the cell‐free supernatant of Y9 and subjected to repetitive column chromatography and preparative high performance liquid chromatography (HPLC). Based on data from instrumental analyses, the metabolites were identified as surfactin isomers consisting of C₁₄[Leu₇], C₁₄[Val₇] and C₁₅[Leu₇]. The isomers with leucine showed higher insecticidal activity than the isomers with valine. Y9 was capable of producing biosurfactants as insecticidal metabolites against M. persicae, and showed potential for use as a biocontrol agent in an integrated pest management program.