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Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea

Author:
Horáček, J., Švábová, L., Šarhanová, P., Lebeda, A.
Source:
Biologia plantarum 2013 v.57 no.1 pp. 133-138
ISSN:
0006-3134
Subject:
DNA, Fusarium solani, Pisum sativum, cultivars, flow cytometry, fusaric acid, pathogens, peas, random amplified polymorphic DNA technique
Abstract:
Pea (Pisum sativum L.) somaclones of cultivars Adept, Komet and Bohatýr were obtained after selection in vitro with Fusarium solani filtrate and fusaric acid (FA). R2 regenerants were analysed by random amplification of polymorphic DNA (RAPD; OPAB4, P-14, UBC-556) and inter-retrotransposon amplification polymorphism (IRAP; Ogre) markers. Marker UBC-556 showed different banding patterns for each cultivar, but without specific bands for selected and control plants. Markers OPAB4, P14 and Ogre were useful for clear discrimination between selected and non-selected variants of all three cultivars. Flow cytometry analysis proved the same genome size of selected and non-selected pea lines. Therefore in vitro selection by pathogen derived agents could be the efficient method for obtaining of pea somaclones with increased resistance to F. solani.
Agid:
588295