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LecRK‐V, an L‐type lectin receptor kinase in Haynaldia villosa, plays positive role in resistance to wheat powdery mildew

Zongkuan Wang, Jiangyue Cheng, Anqi Fan, Jia Zhao, Zhongyu Yu, Yingbo Li, Heng Zhang, Jin Xiao, Faheem Muhammad, Haiyan Wang, Aizhong Cao, Liping Xing, Xiue Wang
Plant biotechnology journal 2018 v.16 no.1 pp. 50-62
Dasypyrum villosum, Triticum aestivum, adults, callus, chitin, culms, diploidy, disease resistance, genes, genetically modified organisms, haustoria, hypersensitive response, leaves, lectins, pathogen-associated molecular patterns, pathogens, powdery mildew, protein kinases, receptors, roots, seedlings, signal transduction, wheat
Plant sense potential microbial pathogen using pattern recognition receptors (PRRs) to recognize pathogen‐associated molecular patterns (PAMPs). The Lectin receptor‐like kinase genes (LecRKs) are involved in various cellular processes mediated by signal transduction pathways. In the present study, an L‐type lectin receptor kinase gene LecRK‐V was cloned from Haynaldia villosa, a diploid wheat relative which is highly resistant to powdery mildew. The expression of LecRK‐V was rapidly up‐regulated by Bgt inoculation and chitin treatment. Its transcript level was higher in the leaves than in roots, culms, spikes and callus. Single‐cell transient overexpression of LecRK‐V led to decreased haustorium index in wheat variety Yangmai158, which is powdery mildew susceptible. Stable transformation LecRK‐V into Yangmai158 significantly enhanced the powdery mildew resistance at both seedling and adult stages. At seedling stage, the transgenic line was highly resistance to 18 of the tested 23 Bgt isolates, hypersensitive responses (HR) were observed for 22 Bgt isolates, and more ROS at the Bgt infection sites was accumulated. These indicated that LecRK‐V confers broad‐spectrum resistance to powdery mildew, and ROS and SA pathways contribute to the enhanced powdery mildew resistance in wheat.