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Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection

Author:
Elise A. Lamont, Harish K. Janagama, Joao Ribeiro-Lima, Lucy Vulchanova, Meetu Seth, My Yang, Kiran Kurmi, W. Ray Waters, Tyler Thacker, Srinand Sreevatsan
Source:
Journal of Clinical Microbiology 2014 v.52 no.2 pp. 536-543
ISSN:
1098-660X
Subject:
Mycobacterium avium subsp. paratuberculosis, Mycobacterium bovis, Mycobacterium kansasii, bacterial proteins, binding proteins, biomarkers, blood proteins, blood serum, bovine tuberculosis, cattle, data collection, diagnostic techniques, disease diagnosis, monitoring, pathogens, peptides, vitamin D
Abstract:
Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating Mycobacterium bovis infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (PLoS One 4:e5478, 2009, doi:10.1371/journal.pone.0005478) identified 32 host peptides that specifically increased in the blood serum of M. bovis-infected animals). In the current study, 16 M. bovis proteins were discovered in the blood serum proteomics data sets. A large-scale validation analysis was undertaken for selected host and M. bovis proteins using a cattle serum repository containing M. bovis (n = 128), Mycobacterium kansasii (n = 10), and Mycobacterium avium subsp. paratuberculosis (n = 10), cases exposed to M. bovis (n = 424), and negative controls (n = 38). Of the host biomarkers, vitamin D binding protein (VDBP) showed the greatest sensitivity and specificity for M. bovis detection. Circulating M. bovis proteins, specifically polyketide synthetase 5, detected M. bovis-infected cattle with little to no seroreactivity against M. kansasii- and M. avium subsp. paratuberculosis-infected animals. These data indicate that host and pathogen serum proteins can serve as reliable biomarkers for tracking M. bovis infection in animal populations.
Agid:
58865
Handle:
10113/58865