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Synthetic green fluorescent protein chromophore analogues with a positive charge at the phenyl-like group

Author:
Zhang, Zhao-Yi, Sung, Robert, Sung, Kuangsen
Source:
Amino acids 2018 v.50 no.1 pp. 141-147
ISSN:
0939-4451
Subject:
Lewis acids, absorption, carbon, deuterium oxide, emissions, energy, fluorescence, green fluorescent protein, half life, hydrolysis, moieties
Abstract:
Synthetic green fluorescent protein (GFP) chromophore analogues with a positive charge at the phenyl-like group have the highly electrophilic amidine carbon, smaller LUMO–HOMO energy gap, red-shifted electronic absorptions and fluorescent emissions, and accelerated E-Z thermoisomerization rates. They are water-labile and their hydrolysis results in ring-opening of the imidazolinone moiety with a half life around 25–37 h in D₂O at 25 °C.