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AU-1 from Agavaceae plants downregulates the expression of glycolytic enzyme phosphoglycerate mutase

Author:
Fujino, Tomofumi, Yokosuka, Akihito, Ichikawa, Harutaka, Oshima, Toshiyuki, Mimaki, Yoshihiro, Hayakawa, Makio
Source:
Natural medicines 2018 v.72 no.1 pp. 342-346
ISSN:
1340-3443
Subject:
Asparagaceae, carcinogenesis, cell lines, glycolysis, saponins
Abstract:
The spirostanol saponin AU-1 from Agavaceae plants stimulates the expression of the glycolytic enzyme phosphoglycerate mutase (PGAM) in ACHN cells. We hypothesized that this may arise from the downregulation of the NAD⁺-dependent deacetylase SIRT1. In this article, we showed that, unlike in renal adenocarcinoma cells, AU-1 does not affect the expression of SIRT1 in the normal renal cell-derived cell line HK-2. Consistent with the lack of a downregulation of SIRT1, AU-1 did not upregulate, but rather decreased PGAM expression. Moreover, AU-1 inhibited the increase in PGAM levels that results from the knock-down of SIRT1. Our results suggest that AU-1 may prevent carcinogenesis caused by increased cellular PGAM.
Agid:
5889774