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Simultaneous analysis of carbohydrates, polyols and amines in urine samples using chemical ionization gas chromatography with tandem mass spectrometry

Author:
Kubinec, Róbert, Kotora, Peter, Ferenczy, Viktória, Blaško, Jaroslav, Podolec, Peter, Hengerics Szabó, Alexandra, Behúlová, Darina, Bierhanzl, Václav, Čabala, Radomír, Stuchlík, Stanislav, Filipiak, Wojciech, Thắng, Ngô Mạnh
Source:
Journal of separation science 2018 v.41 no.2 pp. 449-458
ISSN:
1615-9306
Subject:
amines, amino acids, derivatization, detection limit, galactosemia, gas chromatography, glucose, ionization, mannitol, metabolites, neonates, tandem mass spectrometry, urine
Abstract:
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O‐bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL‐¹ for mannitol to 4.7 mg/L for glucose.
Agid:
5891019