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Target‐guided isolation of polar antioxidants from Abelmoschus esculentus (L). Moench by high‐speed counter‐current chromatography method coupled with wavelength switching and extrusion elution mode

Author:
Wang, Ranhao, Liu, Qi, Wu, Zhiliang, Wang, Meiling, Chen, Xiaoqing
Source:
Journal of separation science 2016 v.39 no.20 pp. 3983-3989
ISSN:
1615-9306
Subject:
2,2-diphenyl-1-picrylhydrazyl, Abelmoschus esculentus, antioxidant activity, antioxidants, bioactive compounds, countercurrent chromatography, extrusion, mass spectrometry, nuclear magnetic resonance spectroscopy, screening, tryptophan, ultraviolet-visible spectroscopy, wavelengths
Abstract:
An off‐line two‐dimensional high‐speed counter‐current chromatography strategy combined with the wavelength switching technique and extrusion elution mode was successfully developed and applied to the isolation of polar antioxidants from Abelmoschus esculentus (L).Moench. Target‐guided by the result of 2,2‐diphenyl‐1‐picrylhydrazyl screening assay, four antioxidants were obtained with purities over 90% through orthogonal high‐speed counter‐current chromatography separation. UV spectroscopy, mass spectrometry and ¹H NMR spectroscopy were employed to identify their structures, which were assigned as l‐tryptophan, quercetin‐3‐O‐sophoroside, 5,7,3′,4′‐tetrahydroxyflavonol‐3‐O‐[β‐d‐rhamnopyranosil‐(1→2)]‐β‐d‐glucopyranoside, and quercetin‐3‐O‐glucoside. Each monomer exhibited significant antioxidant activity. The results demonstrated that proposed method could be an effective approach to isolate bioactive compounds from complex natural products.
Agid:
5891103