Jump to Main Content
Inactivation of virginiamycin by Aureobasidium pullulans
- Leathers, TimothyD., Rich, JosephO., Nunnally, MelindaS., Anderson, AmberM.
- Biotechnology letters 2018 v.40 no.1 pp. 157-163
- Aureobasidium pullulans, bioremediation, ethanol fuels, ethanol production, feeds, grains, high performance liquid chromatography, laccase, phylogeny, virginiamycin
- OBJECTIVE: To test the inactivation of the antibiotic, virginiamycin, by laccase-induced culture supernatants of Aureobasidium pullulans. RESULTS: Fourteen strains of A. pullulans from phylogenetic clade 7 were tested for laccase production. Three laccase-producing strains from this group and three previously identified strains from clade 5 were compared for inactivation of virginiamycin. Laccase-induced culture supernatants from clade 7 strains were more effective at inactivation of virginiamycin, particularly at 50 °C. Clade 7 strain NRRL Y-2567 inactivated 6 µg virginiamycin/ml within 24 h. HPLC analyses indicated that virginiamycin was degraded by A. pullulans. CONCLUSIONS: A. pullulans has the potential for the bioremediation of virginiamycin-contaminated materials, such as distiller’s dry grains with solubles (DDGS) animal feed produced from corn-based fuel ethanol production.