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Antimicrobial Activity of N-Halamine–Coated Materials in Broiler Chicken Houses

Ren, Tian, Qiao, Mingyu, Zhang, Lei, Weese, Jean, Huang, Tung-Shi, Ren, Xuehong
Journal of food protection 2018 v.81 no.2 pp. 195-201
Campylobacter jejuni, Salmonella Typhimurium, aluminum, anti-infective agents, antimicrobial properties, aqueous solutions, bacteria, broiler chickens, chlorine, microbial load, plastics, poultry housing, poultry production, stainless steel, storage time, thiosulfates, titration, wood
The antimicrobial activity of 1-chloro-2,2,5,5-tetramethyl-4-imidazoidinone (MC), a nonbleaching N-halamine compound, was investigated on materials commonly used in broiler production, including stainless steel, galvanized metal, aluminum, plastic, and pressure-treated wood. MC aqueous solutions at 0.02, 0.04, and 0.06% were challenged with Salmonella Typhimurium and Campylobacter jejuni at 6 log CFU/mL, resulting in complete inactivation of both bacteria in 30 min with 0.06% MC. Follow-up experiments were performed using test materials treated with 0.1 and 1% MC and challenged with Salmonella Typhimurium and C. jejuni at 6 log CFU per coupon. Stability of MC on the various surfaces of testing materials was assessed, and the chlorine content of the materials was measured using iodometric thiosulfate titration over a 4-week period. Antimicrobial activities were evaluated by a sandwich test on each sampling day during 4 weeks of storage. On the samples treated with 1% MC, bacteria at 6 log CFU per coupon were completely inactivated within 2 h of contact time. The antimicrobial activity extended to 4 weeks, and the active chlorine atoms in the treated materials decreased from the initial 10(16) to 10(15) atoms per cm2. Overall, MC had high stability and long-lasting antimicrobial activity, which suggests that MC has high potential for use as a novel antimicrobial agent to lower the microbial load on broiler house materials.