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Polyunsaturated fatty acids production with a solid-state column reactor
- Jang, Hung-Der, Yang, Shang-Shyng
- Bioresource technology 2008 v.99 no.14 pp. 6181-6189
- Mortierella, aeration, alpha-linolenic acid, arachidonic acid, carbon, eicosapentaenoic acid, linoleic acid, nitrogen, pH, rice bran, solid state fermentation, temperature, water content
- To investigate the potential production of polyunsaturated fatty acids (PUFAs), a solid-state column reactor of rice bran with Mortierella alpina was used. The optimal conditions for PUFAs production were rice bran supplementation with 3.75% (ww⁻¹) nitrogen source at initial moisture content 57%, initial pH 6-7, aeration, and incubation at 20°C for 5 days and then at 12°C for 7 days. Each gram of substrate carbon yielded 127mg of total PUFAs, 12mg of eicosapentaenoic acid (EPA), 6mg of arachidonic acid (AA), 5mg of α-linolenic acid (ALA), and 117mg of linoleic acid (LA) after 12 days incubation. Aeration enhanced the productions of AA, EPA, and total PUFAs. Supplementation of the nitrogen source on the fourth day and then a shift to lower temperature on the fifth day increased EPA production.