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Abundance of antibiotic resistance genes and bacterial community composition in wild freshwater fish species

Marti, Elisabet, Huerta, Belinda, Rodríguez-Mozaz, Sara, Barceló, Damià, Marcé, Rafael, Balcázar, Jose Luis
Chemosphere 2018 v.196 pp. 115-119
Cyprinus carpio, Salmo trutta, anthropogenic activities, antibiotic resistance, antibiotic resistance genes, antibiotics, aquatic environment, bacterial communities, community structure, ecosystems, freshwater fish, mucus, pollution, ribosomal RNA, sediments, sequence analysis
This study was aimed to determine the abundance of four antibiotic resistance genes (blaTEM, ermB, qnrS and sulI), as well as bacterial community composition associated with the intestinal mucus of wild freshwater fish species collected from the Foix and La Llosa del Cavall reservoirs, which represent ecosystems with high and low anthropogenic disturbance, respectively. Water and sediments from these reservoirs were also collected and analyzed to determine the pollution level by antibiotics. The blaTEM gene was only detected in brown trout and Ebro barbel, which were collected from La Llosa del Cavall reservoir. In contrast, the sulI and qnrS genes were only detected in common carp, which were collected from the Foix reservoir. Although the ermB gene was also detected in common carp, the values were below the limit of quantification. Likewise, water and sediment samples from the Foix reservoir had higher concentrations and more classes of antibiotics than those from La Llosa del Cavall. Pyrosequencing analysis of 16S rRNA genes revealed significant differences in bacterial communities associated with the intestinal mucus of fish species. Therefore, these findings suggest that anthropogenic activities are not only increasing the pollution of aquatic environments, but also contributing to the emergence and spread of antibiotic resistance in organisms that inhabit such environments.