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Hepatoprotective effect of Aegle marmelos augmented with piperine co-administration in paracetamol model

Rathee, Deepti, Kamboj, Anjoo, Sachdev, Rajneesh Kant, Sidhu, Shabir
Revista Brasileira de Farmacognosia 2018 v.28 no.1 pp. 65-72
Aegle marmelos, acetaminophen, alanine transaminase, alkaline phosphatase, anti-inflammatory activity, aspartate transaminase, bilirubin, biomarkers, blood serum, catalase, dose response, glucose 6-phosphate, glutathione, glutathione peroxidase, glutathione transferase, glutathione-disulfide reductase, hepatoprotective effect, hepatotoxicity, interleukin-10, lactate dehydrogenase, leaf extracts, liver, malondialdehyde, models, oxidative stress, rats, silymarin, superoxide dismutase, tumor necrosis factor-alpha
The current study explored hepatoprotective effect of Aegle marmelos (L.) Corrêa, Rutaceae, leaves extract. Potentiation of A. marmelos hepatoprotective effect with piperine co-administration was also explored. Wistar rats were randomly divided into seven groups: (i) normal control, (ii) paracetamol group, (iii) silymarin group, (iv) extract-25 group (25mg/kg body), (v) extract-50 group: (50mg/kg), (vi) extract-100 group (100mg/kg) and (vii) extract-25+piperine group. Hepatotoxicity was induced by administering paracetamol orally in a dose of 400mg/kg for seven days. The drugs were administered 30min prior to paracetamol administration and continued for seven days. Animals were ‘sacrificed’ at the end of treatment and serum was collected for evaluating alkaline phosphatase, bilirubin, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase IL-10 and TNF-α levels. Liver homogenates were used for determination of oxidative stress (malondialdehyde, reduced glutathione, superoxide dismutase, catalase, glutathione reductase, GSH-S-transferase, glutathione peroxidase and glucose-6-phosphate dehydrogenase). Serum biochemical markers were significantly higher in paracetamol group as compared to normal control group. Significant increase in oxidative stress parameters and inflammatory mediators was also observed. Treatment with A. marmelos curtailed the toxic effects of paracetamol in a dose dependent fashion. 100mg/kg dose of A. marmelos was found to be most hepatoprotective. The results of extract-100 group were comparable to silymarin group. Low dose of A. marmelos i.e., 25mg/kg was combined with piperine to evaluate potentiation of hepatoprotective effects of A. marmelos. Piperine co-administration potentiated the hepatoprotective effects, because the combination group results were comparable to high dose A. marmelos group. A. marmelos exerts hepatoprotective activity through its antioxidant and anti-inflammatory properties which was enhanced by piperine.