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Xanthorrhizol contents, α-glucosidase inhibition, and cytotoxic activities in ethyl acetate fraction of Curcuma zanthorrhiza accessions from Indonesia

Nurcholis, Waras, Munshif, Ahmad Ajruddin, Ambarsari, Laksmi
Revista Brasileira de Farmacognosia 2018 v.28 no.1 pp. 44-49
Artemia, Curcuma zanthorrhiza, alpha-glucosidase, antineoplastic activity, breeding programs, cytotoxicity, enzyme inhibition, ethyl acetate, high performance liquid chromatography, inhibitory concentration 50, lethal concentration 50, metabolites, mortality, traditional medicine, Indonesia
Curcuma zanthorrhiza Roxb., Zingiberaceae, a species from Indonesia with xanthorrhizol as the major metabolite, has been used as a folk medicine in several of pharmacological activities. This work aimed to evaluate the xanthorrhizol contents, α-glucosidase inhibition, and cytotoxic activities in ethyl acetate fraction from accessions of C. zanthorrhiza. High-performance liquid chromatography investigated xanthorrhizol content with the standard. Pharmacological activities were evaluated by inhibition of α-glucosidase, the brine shrimp lethality test, and anticancer activity. The ethyl acetate fraction yield varied from 8.24% (Karanganyar) to 13.13% (Sukabumi). The xanthorrhizol contents were found to be in the range 43.55% to 47.99% with Ngawi and Wonogiri promising accessions having the lowest and highest value, respectively. IC50 value for α-glucosidase inhibition ranged from 339.05μg/ml (Karanganyar) to 455.01μg/ml (Ngawi). LC50 value for cytotoxic activities ranged from 33.25μg/ml (Ngawi) to 42.28μg/ml (Karanganyar) in brine shrimp lethality test, 3.10μg/ml (Karanganyar) to 9.85μg/ml (cursina-III) in Vero cell, and 1.17μg/ml (Ngawi) to 6.83μg/ml (Sukabumi) in MCF-7 cell. In this study, C. zanthorrhiza accessions have a high in xanthorrhizol contents and cytotoxic activities that showed a high potential of studied accessions for breeding programs on a commercial scale.