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Xanthorrhizol contents, α-glucosidase inhibition, and cytotoxic activities in ethyl acetate fraction of Curcuma zanthorrhiza accessions from Indonesia

Author:
Nurcholis, Waras, Munshif, Ahmad Ajruddin, Ambarsari, Laksmi
Source:
Revista Brasileira de Farmacognosia 2018 v.28 no.1 pp. 44-49
ISSN:
0102-695X
Subject:
Artemia, Curcuma zanthorrhiza, alpha-glucosidase, antineoplastic activity, breeding programs, cytotoxicity, enzyme inhibition, ethyl acetate, high performance liquid chromatography, inhibitory concentration 50, lethal concentration 50, metabolites, mortality, traditional medicine, Indonesia
Abstract:
Curcuma zanthorrhiza Roxb., Zingiberaceae, a species from Indonesia with xanthorrhizol as the major metabolite, has been used as a folk medicine in several of pharmacological activities. This work aimed to evaluate the xanthorrhizol contents, α-glucosidase inhibition, and cytotoxic activities in ethyl acetate fraction from accessions of C. zanthorrhiza. High-performance liquid chromatography investigated xanthorrhizol content with the standard. Pharmacological activities were evaluated by inhibition of α-glucosidase, the brine shrimp lethality test, and anticancer activity. The ethyl acetate fraction yield varied from 8.24% (Karanganyar) to 13.13% (Sukabumi). The xanthorrhizol contents were found to be in the range 43.55% to 47.99% with Ngawi and Wonogiri promising accessions having the lowest and highest value, respectively. IC50 value for α-glucosidase inhibition ranged from 339.05μg/ml (Karanganyar) to 455.01μg/ml (Ngawi). LC50 value for cytotoxic activities ranged from 33.25μg/ml (Ngawi) to 42.28μg/ml (Karanganyar) in brine shrimp lethality test, 3.10μg/ml (Karanganyar) to 9.85μg/ml (cursina-III) in Vero cell, and 1.17μg/ml (Ngawi) to 6.83μg/ml (Sukabumi) in MCF-7 cell. In this study, C. zanthorrhiza accessions have a high in xanthorrhizol contents and cytotoxic activities that showed a high potential of studied accessions for breeding programs on a commercial scale.
Agid:
5895609