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A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody

Mary, J.Asnet, Jittmittraphap, Akanitt, Chattanadee, Siriporn, Leaungwutiwong, Pornsawan, Shenbagarathai, R.
Virus genes 2018 v.54 no.1 pp. 25-32
Dengue virus, animal pathogens, dengue, glycoproteins, host-pathogen relationships, immune response, immunization, keyhole limpet hemocyanin, neutralization, neutralizing antibodies, pathogenicity, polyclonal antibodies, rabbits, serotypes, synthetic peptides, vaccines
Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and conjugated with keyhole limpet hemocyanin. A standard immunization protocol was followed for the production of polyclonal antibodies by immunizing rabbits against the synthetic peptide. The immune response elicited high-titer polyclonal antibodies with the reactivity of the anti-peptide antibody against both synthetic peptide and four serotypes of DENV confirmed by DOT-ELISA. Neutralizing activity of anti-peptide antibody was found to be cross-reactive and effective resulting in 60% reduction of infectivity at 1:200 dilution in all four serotypes of DENV. Our findings have the potential to further improve our understanding of virus–host interactions and provide new insights into neutralizing antibodies and could also be used as a drug target.