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Effects of average molar weight, crystallinity, and hemicelluloses content on the enzymatic hydrolysis of sisal pulp, filter paper, and microcrystalline cellulose

Kaschuk, Joice Jaqueline, Frollini, Elisabete
Industrial crops and products 2018 v.115 pp. 280-289
cellulose, cellulosic materials, crystal structure, enzymatic hydrolysis, glucose, hemicellulose, high performance liquid chromatography, molecular weight, pulp, scanning electron microscopy, sisal
Properties of cellulosic materials, such as average molar mass (MMvis), crystallinity index and hemicelluloses content may influence the efficiency of the enzymatic conversion of cellulose to glucose. Studies on the simultaneous influence of these parameters have been scarcely found in the literature. In the present study, the conversion of cellulose to glucose was investigated using cellulosic materials with different MMvis, crystallinity, and hemicelluloses content: microcrystalline cellulose (MCC, MMvis = 22104 g mol−1, CI = 79%, no hemicelluloses detected), sisal pulp (SP, MMvis = 94618 g mol−1, CI = 66%, hemicelluloses content = 2.6%) and filter paper (FP, MMvis = 98530 g mol−1, CI = 63%, hemicelluloses content = 19.2%). During the reactions, aliquots were withdrawn and, in addition to the liquors, the unreacted cellulosic materials were evaluated by MMvis, CI, SEM, length and thickness, which can help further understand the reaction as a whole. The liquors were characterized by high-performance liquid chromatography (HPLC) and Miller's method (or DNS). The highest yield for the conversion of cellulose to glucose was observed for SP (88%), followed by MCC (64%) and FP (52%). The results indicated that the presence of high hemicelluloses content (FP) had a more significant interference effect than high crystallinity (MCC).