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Bacterial community evolutions driven by organic matter and powder activated carbon in simultaneous anammox and denitrification (SAD) process

Ge, Cheng-Hao, Sun, Na, Kang, Qi, Ren, Long-Fei, Ahmad, Hafiz Adeel, Ni, Shou-Qing, Wang, Zhibin
Bioresource technology 2018 v.251 pp. 13-21
Planctomycetes, Proteobacteria, anaerobic ammonium oxidation, bacterial communities, bioreactors, carbon, chemical oxygen demand, databases, denitrification, denitrifying microorganisms, fouling, genes, high-throughput nucleotide sequencing, organic matter, quantitative polymerase chain reaction
A distinct shift of bacterial community driven by organic matter (OM) and powder activated carbon (PAC) was discovered in the simultaneous anammox and denitrification (SAD) process which was operated in an anti-fouling submerged anaerobic membrane bio-reactor. Based on anammox performance, optimal OM dose (50 mg/L) was advised to start up SAD process successfully. The results of qPCR and high throughput sequencing analysis indicated that OM played a key role in microbial community evolutions, impelling denitrifiers to challenge anammox’s dominance. The addition of PAC not only mitigated the membrane fouling, but also stimulated the enrichment of denitrifiers, accounting for the predominant phylum changing from Planctomycetes to Proteobacteria in SAD process. Functional genes forecasts based on KEGG database and COG database showed that the expressions of full denitrification functional genes were highly promoted in RC, which demonstrated the enhanced full denitrification pathway driven by OM and PAC under low COD/N value (0.11).