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Algal-bacterial cooperation improves algal photolysis-mediated hydrogen production

Ban, Shidong, Lin, Weitie, Wu, Fangyan, Luo, Jianfei
Bioresource technology 2018 v.251 pp. 350-357
Chlamydomonas reinhardtii, Chlorella, Pseudomonas, Scenedesmus, algae, bacteria, chlorophyll, coculture, culture media, dissolved oxygen, headspace analysis, hydrogen, hydrogen production, microbial growth, oxygen, protein content, starch
In this study, bacterium Pseudomonas sp. strain D was proved to be the main partner assisting Chlamydomonas reinhardtii in improving photolysis-mediated H₂ production and a good partner for promoting H₂ production by the green algae Chlorella and Scenedesmus. In strain D partnered algal-bacterial co-culture, the relative O₂ content in the headspace plus the dissolved oxygen in the culture medium were rapidly consumed by bacterial growth, resulting in a completely anaerobic environment that proved suitable for the activation of algal hydrogenase. Moreover, algal-bacterial cooperation was able to slow the reduction of chlorophyll, enhance starch accumulation, and maintain protein content, which are the potential factors whose control provides an opportunity for improving algal H₂ production. This study systematically analyzed the main pathway responsible for H₂ production by algal-bacterial cooperation and the potential mechanisms for improvement, and proposed an efficient and durable algal-bacterial cooperation system for improving photolysis-mediated H₂ production by green algae.