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Application of growth-phase based light-feeding strategies to simultaneously enhance Chlorella vulgaris growth and lipid accumulation

Sun, Yahui, Liao, Qiang, Huang, Yun, Xia, Ao, Fu, Qian, Zhu, Xun, Fu, Jingwei, Li, Jun
Bioresource technology 2018 v.256 pp. 421-430
Chlorella vulgaris, biomass, developmental stages, light intensity, lipids, microalgae
Considering the variations of optimal light intensity required by microalgae cells along with growth phases, growth-phase light-feeding strategies were proposed and verified in this paper, aiming at boosting microalgae lipid productivity from the perspective of light conditions optimization. Experimental results demonstrate that under an identical time-averaged light intensity, the light-feeding strategies characterized by stepwise incremental light intensities showed a positive effect on biomass and lipid accumulation. The lipid productivity (235.49 mg L−1 d−1) attained under light-feeding strategy V (time-averaged light intensity: 225 μmol m−2 s−1) was 52.38% higher over that obtained under a constant light intensity of 225 μmol m−2 s−1. Subsequently, based on light-feeding strategy V, microalgae lipid productivity was further elevated to 312.92 mg L−1 d−1 employing a two-stage based light-feeding strategy V560 (time-averaged light intensity: 360 μmol m−2 s−1), which was 79.63% higher relative to that achieved under a constant light intensity of 360 μmol m−2 s−1.