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Freezing tolerance of chickpea: biochemical and molecular changes at vegetative stage

Arslan, Ö., Eyidoğan, F., Ekmekçi, Y.
Biologia plantarum 2018 v.62 no.1 pp. 140-148
Cicer arietinum, acclimation, chickpeas, cold, cold tolerance, cultivars, enzyme activity, freezing, gene expression, hydrogen peroxide, malondialdehyde, photochemistry, photosystem II, physiological response, pigments, proline, vegetative growth, wild relatives
The aim of this study was to find a correlation between the freezing tolerance of three chickpea (Cicer arietinum L.) cultivars (İnci, Işık-05, and Sarı-98) and their wild relative C. echinospermum and physiological responses. Chickpea plants (15-d-old) were subjected to cold acclimation (CA) (10 °C for 7 d), freezing (-3 or -5 °C for 2 h), and subsequent rewarming (25 °C for 7 d). In two separate experiments with three replications, we determined growth, water status, photosystem 2 photochemical activity, photosynthetic pigments, H₂O₂, malondialdehyde, and proline content, relative leakage ratio, antioxidant enzyme activities, and gene expressions in cultivars different in freezing tolerance. Freezing temperatures adversely affected all the physiological parameters of all cultivars. Rewarming did not lead to complete recovery. The cultivar İnci was more tolerant to the freezing temperatures than others.