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A method for determining the severity of sudden death syndrome in soybeans
- Cui, D., Zhang, Q., Li, M., Slaminko, T., Hartman, G. L.
- ARS USDA Submissions 2014 v.57 no.2 pp. 671
- Fusarium, Glycine max, breeding lines, cameras, color, cultivars, death, foliar diseases, fungal diseases of plants, fungi, greenhouse experimentation, image analysis, leaf area, leaves, lesions (plant), solar radiation, soybeans
- Sudden death syndrome (SDS), caused by the fungus Fusarium virguliforme, is a widespread mid- to late season soybean disease with distinctive foliar symptoms that in some extreme cases may cause nearly 100% yield loss. This article reports on the development of an image analysis method to quantify SDS severity using an RGB camera under natural light in a laboratory environment. An HSI (hue, saturation, and intensity) color space based image processing approach was developed. A lesion index (LI) was defined as the ratio of affected leaf area to healthy area for evaluating SDS severity levels on leaves of infected plants. A method of using the frequency distribution of hue values of whole leaf area to measure the severity of SDS was also investigated. Preliminary results obtained from laboratory-scale validation demonstrated that the developed method could provide a feasible means of recording SDS severity in situ that could potentially be used for high-throughput greenhouse evaluation of breeding lines or commercial soybean cultivars.