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A calcium-stimulated serine peptidase from a true-branching cyanobacterium, Westiellopsis ramosa sp. nov.

Author:
Dubey, Neelam, Singh, Prashant, Bagchi, SuvendraNath
Source:
Physiology and molecular biology of plants 2018 v.24 no.2 pp. 261-273
ISSN:
0971-5894
Subject:
Anabaena variabilis, EDTA (chelating agent), calcium, chelating agents, chromatography, enzyme activity, gelatin, metalloproteinases, nitrogen-fixing cyanobacteria, pH, plasmin, polyacrylamide gel electrophoresis, serine, temperature, trypsin, vegetative cells
Abstract:
Unbranched heterocytous cyanobacteria produce a number of serine peptidases. We have characterized several peptidases in the cell-free extracts of a true-branched N₂-fixing cyanobacterium, Westiellopsis ramosa sp. nov. Upon substrate-gel zymography of intact filaments and heterocytes, five peptidase bands were resolved, whereas in vegetative cells, a single band was discernible. No band was detected in [Formula: see text]-grown cultures suggesting that the peptidases were present under diazotrophic conditions with much of them confined to heterocytes. Using salt precipitation and chromatography, a caseinolytic peptidase, called Wrp49, was purified which also demonstrated fibrinolytic activity. In SDS-PAGE, the purified peptidase was resolved into 17 and 27 kDa fragments. The enzyme in its native state exhibited Mᵣ ≈ 49 kDa, and digested gelatin in a substrate gel at a corresponding position. The enzyme showed amidolytic activity on a plasmin specific substrate, D-Val-Leu-Lys p-nitroanilide. Moreover, a trypsin specific substrate, N-benzoyl-DL-Arg p-nitroanilide was hydrolyzed at an apparent Kₘ = 0.195 mM and Vₘₐₓ = 5 × 10⁻⁷ M s⁻¹. The enzyme was stable in a wide pH and temperature range. While Ca²⁺ stimulated the activity; phenylmethane sulfonyl fluoride, leupeptin, EDTA and chelants were inhibitory. The activity of the EDTA-inactivated enzyme was completely restored upon adding Ca²⁺, suggesting that both compounds competed with each other in modulating the enzyme activity. The enzyme showed similarities with a Ca²⁺ stimulated subtilisin-like serine peptidase of Anabaena variabilis ATCC 29413, but also presented several unique features of metallopeptidases, such as the chelant’s response. Moreover, the N-terminal sequence (MTVENLARTGVGPGWR) did not match with any of the known peptidases.
Agid:
5898200