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Fine-mapping and identifying candidate genes conferring resistance to Soybean mosaic virus strain SC20 in soybean

Karthikeyan, Adhimoolam, Li, Kai, Li, Cui, Yin, Jinlong, Li, Na, Yang, Yunhua, Song, Yingpei, Ren, Rui, Zhi, Haijian, Gai, Junyi
Theoretical and applied genetics 2018 v.131 no.2 pp. 461-476
Glycine max, Soybean mosaic virus, chromosome mapping, chromosomes, crop production, disease resistance, dominant genes, gene segregation, genetic markers, genomics, heterozygosity, inbred lines, interleukins, marker-assisted selection, molecular cloning, parents, phenotype, proteins, quantitative polymerase chain reaction, resistance genes, reverse transcriptase polymerase chain reaction, selfing, sequence analysis, soybeans, China
KEY MESSAGE: The Mendelian gene conferring resistance to Soybean mosaic virus Strain SC20 in soybean was fine-mapped onto a 79-kb segment on Chr.13 where two closely linked candidate genes were identified and qRT-PCR verified. Soybean mosaic virus (SMV) threatens the world soybean production, particularly in China. A country-wide SMV strain system composed of 22 strains was established in China, among which SC20 is a dominant strain in five provinces in Southern China. Resistance to SC20 was evaluated in parents, F₁, F₂ and the F₂:₇ RIL (recombinant inbred line) population derived from a cross between Qihuang-1 (resistant) and NN1138-2 (susceptible). The segregation ratio of resistant to susceptible in the populations suggested a single dominant gene involved in the resistance to SC20 in Qihuang-1. A “partial genome mapping strategy” was used to map the resistance gene on Chromosome 13. Linkage analysis between 178 RILs and genetic markers showed that the SC20-resistance gene located at 3.9 and 3.8 cM to the flanking markers BARCSOYSSR_13_1099 and BARCSOYSSR_13_1185 on Chromosome 13. Subsequently, a residual heterozygote segregating population with 346 individuals was developed by selfing four plants heterozygous at markers adjacent to the tentative SC20-resistance gene; then, the candidate region was delimited to a genomic interval of approximately 79 kb flanked by the new markers gm-ssr_13-14 and gm-indel_13-3. Among the seven annotated candidate genes in this region, two genes, Glyma.13G194700 and Glyma.13G195100, encoding Toll Interleukin Receptor–nucleotide-binding–leucine-rich repeat resistance proteins were identified as candidate resistance genes by quantitative real-time polymerase chain reaction and sequence analysis. The two closely linked genes work together to cause the phenotypic segregation as a single Mendelian gene. These results will facilitate marker-assisted selection, gene cloning and breeding for the resistance to SC20.