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Identification, introgression, and molecular marker genetic analysis and selection of a highly effective novel oat crown rust resistance from diploid oat, Avena strigosa

Rines, Howard W., Miller, Marisa E., Carson, Martin, Chao, Shiaoman, Tiede, Tyler, Wiersma, Jochum, Kianian, Shahryar F.
Theoretical and applied genetics 2018 v.131 no.3 pp. 721-733
Avena strigosa, Puccinia coronata, backcrossing, breeding lines, breeding programs, chlorosis, colchicine, crown rust, cultivars, diploidy, disease resistance, genetic analysis, genetic markers, genotyping, hexaploidy, homozygosity, inflorescences, introgression, leaves, loci, mature plants, necrosis, oats, ovule culture, parentage, parents, plant tissues, single nucleotide polymorphism, virulence
KEY MESSAGE: Oat crown rust is one of the most damaging diseases of oat. We identified a new source of resistance and developed KASP and TaqMan markers for selection in breeding programs. A new highly effective resistance to oat crown rust (Puccinia coronata f. sp. avenae) was identified in the diploid oat Avena strigosa PI 258731 and introgressed into hexaploid cultivated oat. Young plants with this resistance show moderate susceptibility, whereas older plant tissues and adult plants are resistant with no virulent isolates encountered in over 8 years of testing. Resistance was incorporated into hexaploid oat by embryo rescue, colchicine chromosome doubling followed by backcrosses with a hexaploid parent, and selection for stable transmission of resistance. To mitigate flag leaf and panicle chlorosis/necrosis associated with the resistance, crosses were made with derived resistant lines to breeding lines of divergent parentage followed by selection. Subsequently, two F₂ sister lines, termed MNBT1020-1 and MNBT1021-1, were identified in which the chlorosis/necrosis was reduced. These two lines performed well in replicated multi-location state trials in 2015 and 2016 out-yielding all cultivar entries. Segregating F₂:₃ plants resulting from crosses of MNBT lines to susceptible parents were genotyped with the oat 6K SNP array, and SNP loci with close linkage to the resistance were identified. KASP assays generated from linked SNPs showed accurate discrimination of the resistance in derivatives of the resistant MNBT lines crossed to susceptible breeding lines. A TaqMan marker was developed and correctly identified homozygous resistance in over 95% of 379 F₄ plants when rust was scored in F₄:₅ plants in the field. Thus, a novel highly effective resistance and associated molecular markers are available for use in breeding, genetic analysis, and functional studies.