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Expression and characterization of a recombinant porcinized antibody against the E2 protein of classical swine fever virus

Author:
Chen, Shucheng, Li, Su, Sun, Huimin, Li, Yongfeng, Ji, Shengwei, Song, Kun, Zhang, Lingkai, Luo, Yuzi, Sun, Yuan, Ma, Jifei, Liu, Pinghuang, Qiu, Hua-Ji
Source:
Applied microbiology and biotechnology 2018 v.102 no.2 pp. 961-970
ISSN:
0175-7598
Subject:
Classical swine fever virus, antigens, dose response, genes, genetic vectors, glycoproteins, hog cholera, mice, monoclonal antibodies, neutralization, neutralizing antibodies, swine, virion
Abstract:
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious and economically important disease of pigs. The envelope glycoprotein E2 of CSFV is the major antigen that induces neutralizing antibodies and confers protection against CSFV infections. Previously, we developed a murine monoclonal antibody (MAb), HQ06, against the E2 protein of CSFV. To produce the antibody conveniently and stably, the genes coding for the variable regions of the heavy and light chains of HQ06 and constant region genes from the swine antibody were fused and cloned into lentiviral expression vectors to express a recombinant porcinized MAb (rHQ06Sw) in mammalian cells. rHQ06Sw was able to react with the E2 protein or the CSFV virions specifically in different assays. Notably, rHQ06Sw could neutralize CSFV infection in a dose-dependent manner. Taken together, the functional porcinized MAb rHQ06Sw was generated, which can be used to develop novel diagnostic assays or to investigate the structure and functions of the E2 protein.
Agid:
5900352