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Ganoderma lucidum phosphoglucomutase is required for hyphal growth, polysaccharide production, and cell wall integrity

Author:
Hu, Yanru, Li, Mengjiao, Wang, Shengli, Yue, Sining, Shi, Liang, Ren, Ang, Zhao, Mingwen
Source:
Applied microbiology and biotechnology 2018 v.102 no.4 pp. 1911-1922
ISSN:
0175-7598
Subject:
Escherichia coli, Ganoderma lucidum, beta-glucans, cell wall components, cell walls, chitin, fungi, genes, glucose 1-phosphate, glucose 6-phosphate, glycolysis, hyphae, molecular cloning, phosphoglucomutase, transcription (genetics)
Abstract:
Phosphoglucomutase (pgm) is an important enzyme in carbohydrate metabolism that is located at the branching point between glycolysis and the Leloir pathway. pgm catalyzes the reversible conversion reaction between glucose-6-phosphate (Glc-6-P) and glucose-1-phosphate (Glc-1-P). The glpgm gene was cloned in Escherichia coli, and the recombinant pgm protein from Ganoderma lucidum was purified in this study. The activity of native pgm was also detected to demonstrate that this predicted gene was functional in G. lucidum. Interestingly, silencing the glpgm gene in the fungus reduced hyphal growth. Moreover, glpgm silencing was associated with declining extracellular polysaccharide (EPS) production (approximately 20–40% of that in the WT strain) and increasing intracellular polysaccharide (IPS) production (approximately 1.7-fold that in the WT strain). Additionally, in our research, cell wall components were also shown to differ according to the glpgmi strain. Compared with WT, chitin significantly increased by 1.5-fold; however, the content of β-1,3-glucan was observably reduced to 60–70% that of the WT. Further research showed that the cell wall component changes were associated with the transcription of related genes. These findings provide references for further study on the potential physiological function of pgm in G. lucidum.
Agid:
5900436