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Contrasting growth properties of Nocardioides JS614 on threedifferent vinyl halides

Taylor, AnneE., Bottomley, PeterJ., Semprini, Lewis
Applied microbiology and biotechnology 2018 v.102 no.4 pp. 1859-1867
Nocardioides, bromides, carbon, chlorides, energy, ethylene, fluorides, inoculum, metabolism, metabolites, microbial growth, organobromine compounds, reducing agents, toxicity, vinyl chloride
Ethene (ETH)-grown inocula of Nocardioides JS614 grow on vinyl chloride (VC), vinyl fluoride (VF), or vinyl bromide (VB) as the sole carbon and energy source, with faster growth rates and higher cell yields on VC and VF than on VB. However, whereas acetate-grown inocula of JS614 grow on VC and VF after a lag period, growth on VB did not occur unless supplemental ethene oxide (EtO) was present in the medium. Despite inferior growth on VB, the maximum rate of VB consumption by ETH-grown cells was ~ 50% greater than the rates of VC and VF consumption, but Br⁻ release during VB consumption was non-stoichiometric with VB consumption (~ 66%) compared to 100% release of Cl⁻ and F⁻ during VC and VF consumption. Evidence was obtained for VB turnover-dependent toxicity of cell metabolism in JS614 with both acetate-dependent respiration and growth being significantly reduced by VB turnover, but no VC or VF turnover-dependent toxicity of growth was detected. Reduced growth rate and cell yield of JS614 on VB probably resulted from a combination of inefficient metabolic processing of the highly unstable VB epoxide (t₀.₅ = 45 s), accompanied by growth inhibitory effects of VB metabolites on acetate-dependent metabolism. The exact role(s) of EtO in promoting growth of alkene repressed JS614 on VB remains unresolved, with evidence of EtO inducing epoxide consuming activity prior to an increase in alkene oxidizing activity and supplementing reductant supply when VB is the growth substrate.