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Highly sensitive and specific detection of Giardia duodenalis, Entamoeba histolytica, and Cryptosporidium spp. in human stool samples by the BD MAX™ Enteric Parasite Panel
- Parčina, Marijo, Reiter-Owona, Ingrid, Mockenhaupt, FrankP., Vojvoda, Valerija, Gahutu, JeanBosco, Hoerauf, Achim, Ignatius, Ralf
- Parasitology research 2018 v.117 no.2 pp. 447-451
- Cryptosporidium parvum, Entamoeba histolytica, Giardia lamblia, enteritis, false positive results, feces, human resources, laboratory diagnosis, light microscopy, parasites, quantitative polymerase chain reaction
- Detection of intestinal protozoan parasites by light microscopy is cumbersome, needs experienced personnel, and may lack sensitivity and/or specificity as compared with molecular-based stool assays. Here, we evaluated the BD MAX™ Enteric Parasite Panel, i.e., a multiplex real-time PCR assay for simultaneous detection of Giardia duodenalis, Entamoeba histolytica, and cryptosporidia (Cryptosporidium parvum and C. hominis), by examining 200 positive human stool samples (138 × G. duodenalis, 27 × E. histolytica, 35 × Cryptosporidium spp.) and 119 controls including 18 samples with E. dispar. The majority of the samples, i.e., 153/200 (76.5%) positive samples and 66/119 (55.5%) controls, were confirmed by multiplex in-house PCR detecting the same parasites as the BD MAX™ Enteric Parasite Panel. The BD MAX™ assay did not yield false-positive results. Sensitivity and specificity were 97.8% (95% CI, 93.3–99.4%) and 100% (95% CI, 97.4–100%) for G. duodenalis, 100% (95% CI, 84.5–100%) and 100% (95% CI, 98.4–100%) for E. histolytica, and 100% (95% CI, 87.7–100%) and 100% (95% CI, 98.3–100%) for cryptosporidia, and similar data were obtained when only the 219 PCR-confirmed samples were analyzed. Thus, the BD MAX™ Enteric Parasite Panel provides a highly sensitive and specific tool for the laboratory diagnosis of three predominant protozoan parasites causing enteritis.