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Divergent Cryptosporidium parvum subtype and Enterocytozoon bieneusi genotypes in dromedary camels in Algeria

Baroudi, Djamel, Zhang, Hongwei, Amer, Said, Khelef, Djamel, Roellig, DawnM., Wang, Yuanfei, Feng, Yaoyu, Xiao, Lihua
Parasitology research 2018 v.117 no.3 pp. 905-910
Camelus dromedarius, Cryptosporidium parvum, Enterocytozoon bieneusi, camels, feces, genes, genotype, glycoproteins, hosts, internal transcribed spacers, pathogens, protists, ribosomal RNA, sequence analysis, Algeria
Little information is available on the occurrence of the zoonotic protists Cryptosporidium spp. and none on Enterocytozoon bieneusi in camels. This preliminary study was conducted to examine the identity of Cryptosporidium subtypes and E. bieneusi genotypes in dromedary camels in Algeria. A total of 39 fecal specimens were collected from young camels. PCR-sequence analysis of the small subunit rRNA was used to detect and genotype Cryptosporidium spp. Cryptosporidium parvum present was further subtyped by sequence analysis of the 60 kDa glycoprotein gene. PCR-sequence analysis of the ribosomal internal transcribed spacer gene was used to detect and genotype E. bieneusi. Altogether, two and eight of the specimens analyzed were positive for C. parvum and E. bieneusi, respectively. The former was identified as a new subtype that is genetically related to the C. hominis If subtype family, whereas the latter was identified as two related genotypes (Macaque1 and a novel genotype) in the newly assigned E. bieneusi genotype group 8. Although they are not known hosts for C. parvum and E. bieneusi, camels are apparently infected with genetically distinct variants of these pathogens.