Jump to Main Content
Micelle-mediated transport disturbance providing extracellular strategy for alleviating n-butanol stress on Escherichia coli
- Tan, Lin-Rui, Xia, Peng-Fei, Li, Qian, Yuan, Xian-Zheng, Wang, Shu-Guang
- Bioprocess and biosystems engineering 2018 v.41 no.3 pp. 443-447
- Escherichia coli, anionic surfactants, biofuels, butanol, factories, fuel production, micelles, sodium dodecyl sulfate, solvents, toxicity
- One barrier inhibiting further progress in biofuel production is the toxicity of biofuels towards their producers. It is promising to apply gene-based intracellular techniques to engineer better strains with higher organic solvent tolerance. These methods are, however, complex. In the present study, we developed a simple, manageable, and commercial extracellular prototypal strategy to alleviate n-butanol (n-BuOH) stress on Escherichia coli via a micelle-mediated transport disturbance. When the concentration of sodium dodecyl sulfate, a typical anionic surfactant, is high enough to form micelles, n-BuOH will be trapped into/onto the micelles, and the negative charge prevents the n-BuOH from approaching the cells. Our study provides an extracellular strategy to relieve the stress from n-BuOH, and it also exhibits a new angle to advance microbial factories through extracellular routines.