Main content area

Complete genome sequence of two tomato-infecting begomoviruses in Venezuela: evidence of a putative novel species and a novel recombinant strain

Romay, Gustavo, Chirinos, DorysT., Geraud-Pouey, Francis, Gillis, Annika, Mahillon, Jacques, Bragard, Claude
Archives of virology 2018 v.163 no.2 pp. 555-558
Begomovirus, Solanum lycopersicum var. lycopersicum, genome, genomics, leaves, molecular cloning, new species, nucleotide sequences, sequence analysis, tomatoes, viruses, Venezuela
At least six begomovirus species have been reported infecting tomato in Venezuela. In this study the complete genomes of two tomato-infecting begomovirus isolates (referred to as Trujillo-427 and Zulia-1084) were cloned and sequenced. Both isolates showed the typical genome organization of New World bipartite begomoviruses, with DNA-A genomic components displaying 88.8% and 90.3% similarity with established begomoviruses, for isolates Trujillo-427 and Zulia-1084, respectively. In accordance to the guidelines for begomovirus species demarcation, the Trujillo-427 isolate represents a putative new species and the name “Tomato wrinkled mosaic virus” is proposed. Meanwhile, Zulia-1084 represents a putative new strain classifiable within species Tomato chlorotic leaf distortion virus, for which a recombinant origin is suggested.