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Establishment and application of an indirect ELISA for porcine circovirus 3
- Deng, Junhua, Li, Xiangdong, Zheng, DingDing, Wang, Yanwei, Chen, Lingyan, Song, Huanhuan, Wang, Tongyan, Huang, Yuxin, Pang, Wenqiang, Tian, Kegong
- Archives of virology 2018 v.163 no.2 pp. 479-482
- Porcine circovirus, enzyme-linked immunosorbent assay, farms, livestock and meat industry, polymerase chain reaction, serological surveys, swine, viruses, China
- Porcine circovirus type 3 (PCV3) was initially reported in 2016 in the United States of America. Since then, the virus has been detected on swine farms in Poland, South Korea, and China using PCR. However, a serological survey of PCV3 in pig populations has never been conducted. In this study, for the first time, we established an indirect enzyme-linked immunosorbent (ELISA) assay and performed a national retrospective serological survey for PCV3. Our results showed that the PCV3-postive rate increased from 22.35% to 51.88% between 2015 and 2017. The above results suggest PCV3 has spread widely in China with increased positive rates since 2015.