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High genetic diversity of porcine enterovirus G in Schleswig-Holstein, Germany
- Bunke, Jennifer, Receveur, Kerstin, Oeser, AnnChristin, Fickenscher, Helmut, Zell, Roland, Krumbholz, Andi
- Archives of virology 2018 v.163 no.2 pp. 489-493
- DNA primers, Enterovirus G, Porcine enterovirus, RNA, finishing, genetic variation, phylogeny, reverse transcriptase polymerase chain reaction, swine, transcription (genetics), Germany
- Between 2012 and 2015, 495 pooled snout swabs from fattening pigs raised in Schleswig-Holstein, Germany, were screened for the presence of enterovirus G (EV-G) RNA. Nucleic acids were tested in diverse reverse transcription polymerase chain reaction assays applying published oligonucleotide primers specific for the viral protein (VP) 1 and 2/4 encoding regions as well as for 3D polymerase. Phylogenetic analyses of VP1 revealed the presence of 12 EV-G types, three of which had highly divergent sequences suggesting putative new types. Co-circulation of EV-G types was observed in several pigsties. Thus, genetic diversity of EV-G was demonstrated in this small geographic area.