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Molecular characterization of cytosolic cysteine synthase in Mimosa pudica

Author:
Rashid, Md. Harun-Ur-, Iwasaki, Hironori, Oogai, Shigeki, Fukuta, Masakazu, Parveen, Shahanaz, Hossain, Md.Amzad, Anai, Toyoaki, Oku, Hirosuke
Source:
Journal of plant research 2018 v.131 no.2 pp. 319-329
ISSN:
0918-9440
Subject:
Mimosa pudica, bacteria, biosynthesis, complementary DNA, cysteine, cysteine synthase, mimosine, nucleotides, pH, sodium sulfide, sulfur, temperature, thiols
Abstract:
In the cysteine and mimosine biosynthesis process, O-acetyl-L-serine (OAS) is the common substrate. In the presence of O-acetylserine (thiol) lyase (OASTL, cysteine synthase) the reaction of OAS with sulfide produces cysteine, while with 3-hydroxy-4-pyridone (3H4P) produces mimosine. The enzyme OASTL can either catalyze Cys synthesis or both Cys and mimosine. A cDNA for cytosolic OASTL was cloned from M. pudica for the first time containing 1,410 bp nucleotides. The purified protein product from overexpressed bacterial cells produced Cys only, but not mimosine, indicating it is Cys specific. Kinetic studies revealed that pH and temperature optima for Cys production were 6.5 and 50 °C, respectively. The measured Km, Kcat, and Kcat Km⁻¹ values were 159 ± 21 µM, 33.56 s⁻¹, and 211.07 mM⁻¹s⁻¹ for OAS and 252 ± 25 µM, 32.99 s⁻¹, and 130.91 mM⁻¹s⁻¹ for Na₂S according to the in vitro Cys assay. The Cy-OASTL of Mimosa pudica is specific to Cys production, although it contains sensory roles in sulfur assimilation and the reduction network in the intracellular environment of M. pudica.
Agid:
5901547