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Characterization and fine mapping of qPE12, a new locus controlling rice panicle exsertion

Zhao, Chunfang, Zhao, Qingyong, Zhao, Ling, Zhou, Lihui, Chen, Tao, Yao, Shu, Liang, Wenhua, Zhang, Yadong, Wang, Cailin
Euphytica 2018 v.214 no.3 pp. 47
Oryza sativa, agronomic traits, alleles, genetic analysis, genetic background, hybrids, inflorescences, introgression, loci, molecular cloning, phenotype, phenotypic variation, plant height, pollination, rice, substitution lines
Panicle exsertion is an important agronomic trait involved in pollination and hybrid rice seed production. The identification of genes controlling panicle exsertion will play a central role in hybrid rice breeding. C115, a chromosome segment substitution line carrying introgression segments of Nipponbare in genetic background of indica variety 9311, had significantly shortened panicle exsertion, uppermost internode length and plant height. Genetic analysis in F₂ and F₃ populations derived from a cross of C115 and 9311 indicated that the shortened panicle exsertion of C115 was recessive and controlled by a single Medelian factor, and the allele from Nipponbare decreased the phenotypic value. Primary mapping suggested qPE12, the Nipponbare introgression segment locating on the long arm of chromosome 12, was responsible for this phenotypic variation. Based on map-based cloning strategy, fine mapping was carried out with a total of 1130 recessive individuals selected from F₂ and F₃ populations, delimiting qPE12 to a 190-kb region. This result provides important information for isolation of a new gene controlling panicle exsertion in the future.