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Decanethiol functionalized silver nanoparticles are new powerful leishmanicidals in vitro

Author:
Isaac-Márquez, A.P., Talamás-Rohana, P., Galindo-Sevilla, N., Gaitan-Puch, S.E., Díaz-Díaz, N.A., Hernández-Ballina, G.A., Lezama-Dávila, C.M.
Source:
World journal of microbiology & biotechnology 2018 v.34 no.3 pp. 38
ISSN:
0959-3993
Subject:
Leishmania major, Leishmania mexicana, amastigotes, antileishmanials, cutaneous leishmaniasis, cytotoxicity, drugs, in vivo studies, inhibitory concentration 50, macrophages, nanosilver, parasites, patients, promastigotes, propidium, reproduction, staining
Abstract:
We evaluated, for the first time, the leishmanicidal potential of decanethiol functionalized silver nanoparticles (AgNps–SCH) on promastigotes and amastigotes of different strains and species of Leishmania: L. mexicana and L. major isolated from different patients suffering from localized cutaneous leishmaniasis (CL) and L. mexicana isolated from a patient suffering from diffuse cutaneous leishmaniasis (DCL). We recorded the kinetics of promastigote growth by daily parasite counting for 5 days, promastigote mobility, parasite reproduction by CFSE staining’s protocol and promastigote killing using the propidium iodide assay. We also recorded IC₅₀’s of promastigotes and amastigotes, therapeutic index, and cytotoxicity by co-culturing macrophages with AgNps–SCH or sodium stibogluconate (Sb) used as reference drug. We used Sb as a reference drug since it is used as the first line treatment for all different types of leishmaniasis. At concentrations 10,000 times lower than those used with Sb, AgNps–SCH had a remarkable leishmanicidal effect in all tested strains of parasites and there was no toxicity to J774A.1 macrophages since > 85% were viable at the concentrations used. Therapeutic index was about 20,000 fold greater than the corresponding one for Sb treated cells. AgNps–SCH inhibited > 80% promastigote proliferation in all tested parasites. These results demonstrate there is a high leishmanicidal potential of AgNps–SCH at concentrations of 0.04 µM. Although more studies are needed, including in vivo testing of AgNps–SCH against different types of leishmaniasis, they can be considered a potential new treatment alternative.
Agid:
5902932