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Preventive use of berberine in inhibition of lead-induced renal injury in rats

Hasanein, Parisa, Riahi, Hassan
Environmental science and pollution research international 2018 v.25 no.5 pp. 4896-4903
adults, albumins, animal models, antioxidant activity, antioxidants, aqueous solutions, berberine, biomarkers, catalase, creatinine, drinking water, glutathione, histopathology, kidneys, lead, lead acetate, lipid peroxidation, males, malondialdehyde, nephrotoxicity, oxidants, poisoning, protein content, rats, renal function, superoxide dismutase, urea, uric acid
The kidney is one of the main organs affected by lead toxicity. We investigated the effects of berberine on lead-induced nephrotoxicity in adult male Wistar rats. Animals received an aqueous solution of lead acetate (500 mg Pb/L in the drinking water) and/or berberine (50 mg/kg, i.g.) for 8 weeks. Lead caused an increase in malondialdehyde (P < 0.001) and total oxidant status (P < 0.01), and a decrease in reduced glutathione (P < 0.001), catalase (P < 0.01), superoxide dismutase (P < 0.001), and total antioxidant capacity (P < 0.05). Berberine prevented the prooxidant and antioxidant imbalance induced by lead (P < 0.001). Berberine corrected the increased relative kidney weight (P < 0.05) and biomarkers of renal function (creatinine (P < 0.001), urea (P < 0.05), uric acid (P < 0.001), albumin (P < 0.01), and total protein (P < 0.05)) in lead group. It also attenuated lead-induced abnormal renal structure. The results confirmed renoprotective effects of berberine in an animal model of lead-induced nephrotoxicity by molecular, biochemical, and histopathological analysis through inhibiting lipid peroxidation and enhancing antioxidant defense system mechanisms. Therefore, berberine makes a good candidate to protect against the deleterious effect of chronic lead intoxication.