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High responsiveness in de novo shoot organogenesis induction of Passiflora cristalina (Passifloraceae), a wild Amazonian passion fruit species Plant

de Faria, RodrigoBrito, de Carvalho, IlioFealho, Rossi, AnaAparecida Bandini, de Matos, ElyabeMonteiro, Rocha, DiegoIsmael, Paim Pinto, DanielaLopes, Otoni, WagnerCampos, da Silva, MaurecilneLemes
In vitro cellular & developmental biology 2018 v.54 no.2 pp. 166-174
Passiflora, adventitious shoots, benzyladenine, breeding, cotyledons, cytokinins, endosperm, explants, hypocotyls, in vitro studies, kinetin, leaves, light microscopy, micropropagation, organogenesis, passion fruits, seeds, thidiazuron
The aim of the present study was to establish a regeneration system via de novo organogenesis from different types of non-meristematic explants of Passiflora cristalina. Leaf, hypocotyl, root segments, cotyledons, and endosperm of P. cristalina seeds were inoculated in Murashige and Skoog (MS)-basal medium, supplemented with different concentrations of 6-Benzyladenine (BA), Thidiazuron (TDZ), or Kinetin (KIN). BA was found to be the most efficient cytokinin in induction of de novo organogenesis from most the explants used in the study. The highest frequencies of adventitious bud formation in the hypocotyl and cotyledon explants were observed in medium supplemented with 1.0 mg L⁻¹ BA. For leaf and endosperm segments, the best concentration was 2.0 mg L⁻¹ BA; while for root segments, the highest mean values were observed with 1.0 mg L⁻¹ KIN. The different morphogenetic responses obtained from each explant source were characterized using light microscopy. P. cristalina revealed a remarkable organogenic potential, with superior production of adventitious shoots compared with the other Passiflora species evaluated elsewhere. These results will be helpful to establish a reproducible and reliable micropropagation protocol, as well as to implement conservationist and biotechnological-based genetic breeding strategies for this wild Passiflora species.