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Activity of a novel succinate dehydrogenase inhibitor fungicide pyraziflumid against Sclerotinia sclerotiorum

Hou, Yi-Ping, Mao, Xue-Wei, Lin, Shi-Peng, Song, Xiu-Shi, Duan, Ya-Bing, Wang, Jian-Xin, Zhou, Ming-Guo
Pesticide biochemistry and physiology 2018 v.145 pp. 22-28
Sclerotinia sclerotiorum, carbendazim, cross resistance, enzyme inhibitors, fludioxonil, hyphae, leaves, mechanism of action, median effective concentration, membrane permeability, mycelium, oxalic acid, rapeseed, sclerotia, succinate dehydrogenase (quinone), China
Pyraziflumid is a novel member of succinate dehydrogenase inhibitor fungicides (SDHI). In this study, baseline sensitivity of Sclerotinia sclerotiorum (Lib.) de Bary to pyraziflumid was determined using 105 strains collected during 2015 and 2017 from different geographical regions in Jiangsu Province of China, and the average EC50 value was 0.0561 (±0.0263)μg/ml for mycelial growth. There was no cross-resistance between pyraziflumid and the widely used fungicides carbendazim, dimethachlon and the phenylpyrrole fungicide fludioxonil. After pyraziflumid treated, hyphae were contorted with offshoot of top increasing, cell membrane permeability increased markedly, oxalic acid content significantly decreased and mycelial respiration was strongly inhibited. But the number and dry weight of sclerotia did not change significantly. The protective and curative activity test of pyraziflumid suggested that pyraziflumid had great control efficiency against S. sclerotiorum on detached rapeseed leaves, and protective activity was better than curative activity. These results will contribute to us on evaluating the potential of the new SDHI fungicide pyraziflumid for management of diseases caused by S. sclerotiorum and understanding the mode of action of pyraziflumid against S. sclerotiorum.