Main content area

Comparative proteomic analysis of hepatic effects induced by nanosilver, silver ions and nanoparticle coating in rats

Juling, Sabine, Böhmert, Linda, Lichtenstein, Dajana, Oberemm, Axel, Creutzenberg, Otto, Thünemann, Andreas F., Braeuning, Albert, Lampen, Alfonso
Food and chemical toxicology 2018 v.113 pp. 255-266
coatings, complement, data collection, energy metabolism, homeostasis, ions, liver, nanoparticles, nanosilver, oxidative stress, proteins, proteomics, rats, silver, stress response, toxicity, toxicology
The presence of nano-scaled particles in food and food-related products has drawn attention to the oral uptake of nanoparticles and their interactions with biological systems. In the present study, we used a toxicoproteomics approach to allow for the untargeted experimental identification and comparative analysis of cellular responses in rat liver after repeated-dose treatment with silver nanoparticles, ions, and the coating matrix used for particle stabilization.The proteomic analysis revealed treatment-related effects caused by exposure to silver in particulate and ionic form. Both silver species induced similar patterns of signaling and metabolic alterations. Silver-induced cellular alterations comprised, amongst others, proteins involved in metal homeostasis, oxidative stress response, and energy metabolism. However, we discovered that secondary nano-scaled structures were formed from ionic silver. Furthermore, also the coating matrix alone gave rise to the formation of nano-scaled particles.The present data confirm, complement, and extend previous knowledge on silver toxicity in rodent liver by providing a comprehensive proteomic data set. The observation of secondary particle formation from non-particle controls underlines the difficulties in separating particle-, ion-, and matrix coating-related effects in biological systems. Awareness of this issue will support proper evaluation of nanotoxicology-related data in the future.