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Comparative proteomic analysis of hepatic effects induced by nanosilver, silver ions and nanoparticle coating in rats

Author:
Juling, Sabine, Böhmert, Linda, Lichtenstein, Dajana, Oberemm, Axel, Creutzenberg, Otto, Thünemann, Andreas F., Braeuning, Albert, Lampen, Alfonso
Source:
Food and chemical toxicology 2018 v.113 pp. 255-266
ISSN:
0278-6915
Subject:
coatings, complement, data collection, energy metabolism, homeostasis, ions, liver, nanoparticles, nanosilver, oxidative stress, proteins, proteomics, rats, silver, stress response, toxicity, toxicology
Abstract:
The presence of nano-scaled particles in food and food-related products has drawn attention to the oral uptake of nanoparticles and their interactions with biological systems. In the present study, we used a toxicoproteomics approach to allow for the untargeted experimental identification and comparative analysis of cellular responses in rat liver after repeated-dose treatment with silver nanoparticles, ions, and the coating matrix used for particle stabilization.The proteomic analysis revealed treatment-related effects caused by exposure to silver in particulate and ionic form. Both silver species induced similar patterns of signaling and metabolic alterations. Silver-induced cellular alterations comprised, amongst others, proteins involved in metal homeostasis, oxidative stress response, and energy metabolism. However, we discovered that secondary nano-scaled structures were formed from ionic silver. Furthermore, also the coating matrix alone gave rise to the formation of nano-scaled particles.The present data confirm, complement, and extend previous knowledge on silver toxicity in rodent liver by providing a comprehensive proteomic data set. The observation of secondary particle formation from non-particle controls underlines the difficulties in separating particle-, ion-, and matrix coating-related effects in biological systems. Awareness of this issue will support proper evaluation of nanotoxicology-related data in the future.
Agid:
5905216